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. 2022 Jun 13;12:100096. doi: 10.1016/j.ynpai.2022.100096

Fig. 2.

Fig. 2

LD-IL-2 blocks NTG-induced behavioral sensitization through both peripheral IL-10 and TGFβ signaling pathways. (A) Female C57BL/6 mice received i.p. injections of neutralizing antibodies against IL-10 or TGFβ (anti-IL-10 Ab or anti-TGFβ Ab, 200 μg/mouse) before and during repeated NTG administration (n = 4 mice/group). Arrows indicate the injections of antibodies or NTG. On the days that the mouse behaviors were tested, NTG and/or the antibodies were always injected after completing the behavioral tests. Friedman test: p < 0.05 within the anti-TGFβ Ab group (F[3, 12] = 57.24); post hoc Student-Newman-Keuls test: ***p < 0.001, compared with the day 1 and 3 thresholds. Friedman test: p < 0.05 within the anti-IL-10 Ab group (F[3, 12] = 81.60); ###p < 0.001, compared with the day 1 and 3 thresholds. (B) The effect of LD-IL-2 on NTG-induced facial skin hypersensitivity was abolished by anti-IL-10 Ab and anti-TGFβ Ab. Arrows indicate the injection of NTG, LD-IL-2, anti-TGFβ Ab, anti-IL-10 Ab or control IgGs. On the days that the mouse behaviors were tested, drugs were always injected after completing the behavioral tests (n = 6 female C57BL/6 mice/group). In the NTG+IL-2+control IgG group, 3 mice received control IgG for anti-TGFβ Ab and the other 3 received the control IgG for anti-IL-10 Ab. Kruskal-Wallis ANOVA on ranks: p < 0.05 for group (F[3, 160] = 147.7), p < 0.001 for time (F[7, 160] = 68.3), and p < 0.001 for group × time interaction (F[21, 160] = 14.6); post hoc Student-Newman-Keuls test: ***p < 0.001, NTG+IL-2+anti-TGFβ Ab versus the corresponding NTG+IL-2 and NTG+IL-2+control IgG groups; ###p < 0.001, NTG+IL-2+anti-IL-10 Ab versus the corresponding NTG+IL-2 and NTG+IL-2+control IgG groups; §§§p < 0.001, compared with the baseline threshold (day 1) in the NTG+IL-2 group; ^^^p < 0.001, compared with the baseline threshold in the NTG+IL-2+control IgG group;!!!p < 0.001, compared with the baseline threshold in the NTG+IL-2+anti-TGFβ Ab group; †††p < 0.001, compared with the baseline threshold in the NTG+IL-2+anti-IL-10 Ab group.