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Representative traces and images of PACAP-R and CGRP-R TG neurons. (A) The protocol to measure PACAP- and CGRP-evoked intracellular Ca2+ increase and the representative traces of PACAP- and CGRP-induced Ca2+ transients in TG neurons. (B) Representative images of Fura-2-loaded TG neurons before, during and after applications of 50 nM PACAP and 3 μM CGRP, respectively. The arrows indicate the same CGRP-R, PACAP-R and CGRP-R&PACAP-R neurons shown in A, respectively.
