Figure 1.

Labeling procedure

Simplified diagram of four steps involved in semi-automatically labeling live cell movie. In the first step, prepare the raw image and set the parameters of the label tool. In the second step, extract the edge from the raw image. It is inaccurate since edge extraction is performed by a traditional algorithm. Therefore, in the third step, a user needs to fix extracted edge image by connecting fragmented edges or removing incorrect edges. A manually corrected image is shown as an example. Then in the fourth step, a simple flood fill algorithm can segment the cell body region in the fully connected edge image. Scale Bar: 32.5 μm.