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Western blot analysis of cry1Ac7 expression in recombinant clones. (A) Lanes: 1 and 2, E. coli(pKTT) and E. coli(pJTT) carrying the ptac-cry1Ac7 cassette on pKT240 and pJFF350, respectively; 3, P. fluorescens 14; 4, P. fluorescens 14(pKTT); 5, P. fluorescens 14::cry1Ac7 clone 2; 6 to 10, P. fluorescens 14::ptac-cry1Ac7 clones 1 and 2. (B) Lanes: 1, E. coli(pMT7); 2, E. coli(pMT11); 3, H. seropedicae Nal1; 4, H. seropedicae Nal1(pMT7); 5, H. seropedicae Nal1(pMT11).
