TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant genotype/phenotype | Reference or source |
|---|---|---|
| Strains | ||
| E. coli JM105 | F′ traD36 lacIq Δ(lacZ)M15 proAB thi rpsL (Strr) endA sbcB15 (sbcC) hsdR4 (rK− mK+) Δ(lac-proAB) | 38 |
| E. coli S17-1 | recA thi pro hsdR4 (rK− mK+) (RP4-2Tc-Mu-Km-Tn7) Tpr Smr | 30 |
| P. fluorescens Rif1 | Rifr | 8 |
| P. fluorescens 14 | Rifr Nalr | 17 |
| P. fluorescens 14::tox | Rifr Nalr Omegon-Km–cry1Ac7 | 13 |
| H. seropedicae HRC54 | Apr | 3 |
| H. seropedicae Nal1 | Nalr | This work |
| Plasmids | ||
| pKT240 | Kmr Apr | 2 |
| pKK223-3 | Apr ptac rrnB T1 T2 | Pharmacia Biotech |
| pML122 | Gmr Nm PNm | 19 |
| pJFF350 | Kmr | 9 |
| pGH37D-1 | Aprcry1Ac7 | 13 |
| pJTT | Kmr ptac-cry1Ac7 | This work |
| pKTT | Kmr Apr ptac-cry1Ac7 | This work |
| pMT7 | Gmr PNm-cry1Ac7ay | This work |
| pMT11 | Gmrcry1Ac7 | This work |
| pKmM0 | Kmr | 32 |
| pKmM0-tox | Kmrcry1Ac7 | This work |
a
Promoter of the neomycin resistance gene upstream of the cry1Ac7 gene.