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. 2022 Jun 2;25(7):104509. doi: 10.1016/j.isci.2022.104509

Figure 2.

Figure 2

VEGFA promotes HEMEC angiogenesis

(A) HEMECs were stimulated with VEGFA (40 ng/mL) for different time points. Western blotting analysis was used to detect the protein expression of KLF4, MMP-9, PCNA, and Caspase 3.

(B) Densitometric scanning. Data are represented as mean ± SD, n = 3, ∗p < 0.05 vs. 0 h, one-way ANOVA.

(C) VEGFA promoted HEMEC migration by the scratch-wound assay. Statistical data of the results are shown on the right. Data are represented as mean ± SD, n = 3, ∗p < 0.05 vs. 0 h, one-way ANOVA.

(D) Immunofluorescence staining was performed for the expression of Ki67 in HEMECs. Red staining represents Ki67 and blue staining represents the nucleus. Scale bar = 50 μm. Statistical data of the results are shown on the right. Data are represented as mean ± SD, n = 3, ∗p < 0.05 vs. 0 h, one-way ANOVA.