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. 2022 Jun 2;25(7):104509. doi: 10.1016/j.isci.2022.104509

Figure 3.

Figure 3

KLF4 mediates VEGFA-induced endometrial angiogenesis

(A) After HEMECs were infected with Ad-KLF4 adenovirus 24 h and incubated with VEGFA another 12 h, the protein expression of KLF4, MMP-9, PCNA, Caspase 3 were detected by Western blotting.

(B) HEMECs were infected with three KLF4-specific knockdown adenoviruses (sh-KLF4 1, sh-KLF4 2, sh-KLF4 3). Western blotting analysis was performed for KLF4 expression.

(C) After HEMECs were infected with sh-KLF4 2 24 h and stimulated with VEGFA for 12 h, the protein expression of KLF4, MMP-9, PCNA, and Caspase 3 were detected by Western blotting.

(D) HEMECs were treated as (A) and (C). The expression of Ki67 was detected by immunofluorescence staining. Red staining represents Ki67 and blue staining represents the nucleus. Scale bar = 50 μm.

(E) HEMECs were treated as (A) and (C). The migration of HEMECs was detected by Scratch-wound assay.

(F) Statistical data of immunofluorescence staining results in (D). Data are represented as mean ± SD, n = 3, ∗p < 0.05 vs. Ad or sh-con, #p < 0.05 vs. VEGFA, one-way ANOVA.

(G) Statistical data of Scratch-wound assay results in (E), Data are represented as mean ± SD, n = 3, ∗p < 0.05 vs. Ad or sh-con, #p < 0.05 vs. VEGFA, one-way ANOVA.