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. 2022 Apr 28;298(7):101991. doi: 10.1016/j.jbc.2022.101991

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Frequency of short TMHs in selected organelles and organisms.A, representation of the hydrophobic mismatch between Tat subunits and the membrane bilayer. Protein structure was obtained from the Protein Data Bank. B, statistical analysis of the TMH length of proteins across different species. TMH length for each protein was predicted by TMHMM Server, version 2.0, which was then rounded to the nearest integer. Relative frequency in percentages was obtained by calculating the ratio of the number of proteins with predicted TMH at the indicated length to the total number of proteins in the corresponding category (mitochondria, bacteria, chloroplast, and E. coli). N, the total number of entries in each category. Further details are provided in the Experimental procedures section. TMH, transmembrane alpha helix.