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. 2022 Jun 6;14:890231. doi: 10.3389/fnsyn.2022.890231

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Copyright © 2022 Lutz, Bauer, Ioannidis, Schön and Boeckers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western Blot analysis for SHANK3 specificity. (A) Rock-GS, (B) SY-311, (C) SY-302, (D) SY-304, (E) ASC, (F) CS, (G) Fr1+2, (H) Fr1+2+3, and (I) vNterm. Left: The Western Blot shows mouse cortical lysate of WT and KO mice for all antibodies. Same amounts of protein are ensured by β-ACTIN. Right: GFP-SHANK1, GFP-SHANK2, and GFP-SHANK3 were overexpressed in HEK293T cells. Blot were first incubated with the respective SHANK3 antibody and then with an anti-GFP antibody to ensure plasmid expression.