Figure 2.

DRUG-seq reproducibility experimental design. (A) Experimental design depicted by nine plate maps. Within each plate, 14 compounds were plated in 8 doses (3.2 nM to 10 μM) in 3 sectors for a total of 3 replicates per condition per plate (n = 3 per condition). Each sector contained 8 DMSO wells for a total of 24 per plate (n = 24). For each week (or batch) of cells, there were three replicate plates. Each batch of cells was plated on independent days to reflect biological variability. (B) Table depicts compound name, identifier, target, and cluster from Ye et al., 2018 publication. Fourteen compounds were selected to represent a diverse set of MoAs.