| 2× lysis buffer stock—store at room temp | |
| component | volume |
| 1 M Tris-HCl pH8.0 (Life Tech,15568-025) | 1000 μL |
| 2 M KCl (Ambion, AM9640G) | 750 μL |
| 20% of 20% Ficoll PM-400 in H2O | 6000 μL |
| (Sigma, F5415-25ML) | |
| Triton-100 (Sigma, T8787-100ML) | 30 μL |
| H2O | 2220 μL |
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