| RT mix | |
| component | per well |
| 5XRT buffer (ThermoFisher, EP0742) | 0.5 μL |
| 1 M MgCl2 (Ambion, AM9530G) | 0.1 μL |
| regular template switching primer 100 μM | 0.1 μL |
| (Integrated DNA Technologies) | |
| RiboLock RNase inhibitor 40 U/μL | 0.1 μL |
| (ThermoFisher, EO0381) | |
| dNTP 2.5 μM (ThermoFisher, AM8228G) | 0.1 μL |
| maxima RT (ThermoFisher, EP0742) | 0.1 μL |
| H2O (ThermoFisher, AM8228G) | 1.5 μL |
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