| temperature | time | cycles |
| 96 C | preheat and pause | |
| 96 C | 1 min | 1 |
| 98 C | 20 s | 5 |
| 58 C | 4 min | |
| 72 C | 6 min | |
| 98 C | 20 s | 13 |
| 60 C | 30 s | |
| 72 C | 6 min | |
| 72 C | 10 min | 1 |
| 4 C | hold (overnight) |
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