| temperature | time | cycles |
| 72 C | preheat and pause | |
| 72 C | 3 min | 1 |
| 95 C | 30 s | 1 |
| 95 C | 10 s | 15 |
| 55 C | 30 s | |
| 72 C | 30 s | |
| 72 C | 5 min | 1 |
| 4 C | hold |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.