Table 3. Solvent Kinetic Isotope Effects on k_cat for Reactions of TaCPa2E with CDP-Glc and CDP-[2-²H]Glc at Varied Temperatures and pL Values.

substrate	D2Okcat (pL 7.5) 80 °C	D2Okcat (pL 7.0) 60 °C	D2Okcat (pL 7.5) 60 °C	D2Okcat (pL 8.0) 60 °C	D2Okcat (pL 7.5) 20 °C
CDP-Glc	n.d.a	0.97 ± 0.12	1.03 ± 0.13	0.99 ± 0.11	0.49 ± 0.07b
CDP-[2-2H]Glc	0.84 ± 0.03	0.57 ± 0.11	0.57 ± 0.10	0.59 ± 0.10	0.51 ± 0.10

^an.d., not determined.

^bFrom a full Michaelis–Menten analysis performed in these conditions (Figure S3a,b), the ^D₂Ok_cat/K_M was determined as 0.65 (± 0.31).