FIGURE 6.

Chronic HFD/SW induces signaling pathways associated with HCC. (A) Heatmap of expression of HCC‐related and liver‐specific genes in livers from mice fed chow and HFD/SW for 54 weeks. (B) GSEA of gene signatures associated with poor prognosis (73 genes) or good prognosis (113 genes) in human HCC patients compared to those in livers of mice on chow and HFD/SW. (C) GSEA of gene signatures associated with human HCC subclasses S1, S2, and S3 defined by Hoshida et al ²⁵ compared to HFD/SW‐induced HCC at 54 weeks. NES, normalized enrichment score; FDR, false discovery rate. (D) Molecular pathways activated by HFD/SW related to human S1 subclass genes. GSEA, Gene Set Enrichment Analysis; HCC, hepatocellular carcinoma; SW, sugar water.