. 2022 May 25;11(6):2036–2042. doi: 10.1021/acssynbio.1c00525

Table 1. Phage Plaque Yield from the 10-fragment T7 Phage Genome Assembly Reactions^a.

genome topology^b	assembly enzymes^c	replicate 1	replicate 2	replicate 3	average
linear	SapI alone	0	0	0	0
linear	SapI + T4 ligase	81	50	45	59
circular	SapI Alone	0	0	0	0
circular	SapI + T4 ligase	33,600	36,400	38,200	36,100

The table shows the number of phage plaques observed per microliter of assembly reaction transformed into NEB 10-beta cells for three experimental replicates and their average. One microliter of assembly reaction contains approximately 35 ng of DNA.

Assembly reactions were designed to produce two variants of the phage genome with identical sequences that have either blunt termini (linear) or a circular configuration (circular).

Genome assembly reactions were carried out with SapI and T4 DNA ligase. As a control, mock assembly reactions with SapI alone were carried out in parallel.

Table 1. Phage Plaque Yield from the 10-fragment T7 Phage Genome Assembly Reactionsa.

Table 1. Phage Plaque Yield from the 10-fragment T7 Phage Genome Assembly Reactions^a.