. 2022 May 25;11(6):2036–2042. doi: 10.1021/acssynbio.1c00525

Table 2. Phage Plaque Yield from the 52-fragment T7 Phage Genome Assembly Reactions^a.

reaction time (h)^b	assembly enzymes^c	replicate 1	replicate 2	replicate 3	average
5	BsmBI alone	0	0	0	0
5	BsmBI + T4 ligase	13	10	18	14
15	BsmBI alone	0	0	0	0
15	BsmBI + T4 ligase	38	40	45	41

The table shows the number of phage plaques observed per microliter of assembly reaction transformed into NEB 10-beta cells for three experimental replicates and their average.

Assembly reactions to construct the phage genome were cycled between 42 and 16 °C for 5 min at each temperature for 30 cycles (5 h) or 90 cycles (15 h) prior to electroporation.

Genome assembly reactions were carried out with BsmBI and T4 DNA ligase or BsmBI alone as a control.

Table 2. Phage Plaque Yield from the 52-fragment T7 Phage Genome Assembly Reactionsa.

Table 2. Phage Plaque Yield from the 52-fragment T7 Phage Genome Assembly Reactions^a.