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. 2022 Jun 15;50(6):03000605211053703. doi: 10.1177/03000605211053703

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© The Author(s) 2022

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Growth arrest specific 5 (GAS5) overexpression inhibits Th17 cell differentiation. (a) RT-qPCR analysis showing GAS5 expression levels in CD4⁺ T cells transfected with pcDNA3.1 or pcDNA3.1/GAS5. (b) Flow cytometry was used to detect the percentage of Th17 cells and Treg cells in CD4⁺ T cells transfected with pcDNA3.1 or pcDNA3.1/GAS5. (c) The protein levels of RAR-related orphan receptor γt (RORγt) and forkhead box P3 (Foxp3) were detected by western blot in CD4⁺ T cells transfected with pcDNA3.1 or pcDNA3.1/GAS5. (d and e) The protein levels of interleukin (IL)-17 and IL-10 in supernatants were measured by enzyme-linked immunosorbent assays (ELISAs). n = 3; **P < 0.01.