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. 2022 Jun 15;50(6):03000605211053703. doi: 10.1177/03000605211053703

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© The Author(s) 2022

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Growth arrest specific 5 (GAS5) attenuates Th17 differentiation by reducing miR-23a expression. (a) Flow cytometry was used to detect the percentage of Th17 cells in CD4⁺ T cells transfected with pcDNA3.1, pcDNA3.1/GAS5, or pcDNA3.1/GAS5+miR-23a mimic. (b and c) Western blot analysis and enzyme-linked immunosorbent assays (ELISAs) showed the protein expression levels of RAR-related orphan receptor γt (RORγt) and interleukin (IL)-17 in CD4⁺ T cells transfected with pcDNA3.1, pcDNA3.1/GAS5, or pcDNA3.1/GAS5+miR-23a mimic. (d) Flow cytometry was used to detect the percentage of Treg cells in CD4⁺ T cells transfected with pcDNA3.1, pcDNA3.1/GAS5, or pcDNA3.1/GAS5+miR-23a mimic. (e and f) Western blot analysis and ELISAs showed the protein expression levels of forkhead box P3 (Foxp3) and IL-10 in CD4⁺ T cells transfected with pcDNA3.1, pcDNA3.1/GAS5, or pcDNA3.1/GAS5+miR-23a mimic. n = 3; *P < 0.05, **P < 0.01.