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Primer and probe design for circRNA validation by RT‐qPCR. In blue marked the junction site.
| circRNA | ||
|---|---|---|
| circEPB41L2 (hsa_circRNA_0001640) | Forward | GAAGACCAAAACTGTCCAGTGTAAAG |
| Reverse | CACTTCAGACACAGAGCCTACTTCA | |
| Probe | TGACCTGGAGCATAAG | |
| circSOX13 (hsa_circRNA_0004777) | Forward | CAGTGACTGGAAGGAGAGGTTTC |
| Reverse | CTGGGCAGAGATGGGGCT | |
| Probe | AAAGATGTCAAAGGATGTCCATGA | |
| circBNC2 (hsa_circ_0086414) | Forward | GTCTGCACAGTGGCTGGTTG |
| Reverse | GGTGATGATTTCCTCTTCTCGAG | |
| Probe | AGACAGGATGCTGCTG | |
