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. 2022 Jun 20;19:160. doi: 10.1186/s12974-022-02518-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Activation and differentiation of endogenous neural stem cells after transplantation of iPSCs. A Activation and proliferation of endogenous NSCs was detected using BrdU (red)/Nestin (blue) co-immunofluorescence at 14 days after SCI in each group. B, C Double-immunofluorescence staining of NeuN (red) and BrdU to identify the differentiation of newly formed cells towards neurons (arrowheads), or GFAP (blue) and BrdU (red) to identify newly formed astrocytes (asterisk). D Western blotting analysis of Nestin protein expression (*p < 0.05 vs. SCI group, ^###p < 0.001, GFP vs. H-Apln, mean ± SD)