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. 2022 Jun 20;13:82. doi: 10.1186/s40104-022-00723-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Morphological and molecular characterization of s-EVs. A The concentration and size distribution of s-EVs isolated from follicular fluids (FFs) corresponding to high- (HQ) or low-quality (LQ) oocytes analysed by NTA. B Transmission electron microscopy (TEM) representative photos of isolated s-EVs showing the lipid bilayer membrane and the cup-shaped EV particles (black arrows). Scale bar of 500 and 200 nm for the upper left and right photos, respectively, and of 200 and 100 nm for the lower left and right photos, respectively). C Immunoblotting analysis of EV-specific protein markers (ALIX, TSG101, and CD63) and cellular specific protein markers (CytC and ATP5A) in the HQ and LQ EV groups, as well as in filtered FF and follicular cell lysate (FC) as positive and negative controls, respectively. Scale numbers are in kDa. D RNA size distribution from s-EV samples of HQ (sample 1–3) and LQ (sample 4–6) groups analysed with a bioanalyzer (Agilent Technologies)