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. 2022 Jun 20;23:453. doi: 10.1186/s12864-022-08684-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Temperature stress- responsive expression profiles of SoBSKs in leaves from spinach heat- sensitive variety Sp73 and heat- tolerant variety Sp75. The data represented the expression levels of SoBSKs after heat (37 °C) or cold (4 °C) treatments for 0, 2, 4, 12, 24 h, respectively. The results were calculated via the 2^-ΔΔCt method, and the SoARF was used as reference gene to correct the expression levels of SoBSKs. The heatmap of SoBSKs expression based on the RT-qPCR data standardized by log2 conversion. The color bar on the right of the heat map was based on the RT-qPCR data. Red and blue colors indicated up- and down- regulated genes, respectively