TABLE 1.
Pseudomonas strain | Relevant characteristicsa | Soil sample |
---|---|---|
SV1 | Apr Tcr Nalr Cmr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries a 2.3-kb cryptic plasmid | Layer of solidified oil on the surface of a long-distance oil transportation tank; railway station Minsk-South |
SV2 | Apr Tcr Cmr Tln+ mXln+ mTol+ Ben+ C2,3O+ | Same as SV1 |
SV3 | Apr Tcr Cmr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries ∼190-kb plasmid pSVS3 | Sandy soil contaminated with heating oil; southwestern part of Minsk |
SV4 | Tcr Nalr Cmr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries ∼200-kb plasmid pSVS4 | Same as SV3 |
SV5 | Tcr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries ∼150-kb plasmid pSVS5 and a 2.9-kb cryptic plasmid | Railway bed soil contaminated with mixture of lubricants, diesel fuel, and phenol; town of Smorgon, 100 km northwest of Minsk |
SV6 | Tcr Tln+ mXln+ mTol+ Ben+ C2,3O+ Sal+; carries 140-kb plasmid pSVS6 and 10- and 80-kb cryptic plasmids | Grass bed bulk soil contaminated with diesel fuel; central part of Minsk |
SV9 | Apr Tcr Cmr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries 107-kb plasmid pSVS9 | Railway bed soil contaminated with mixture of lubricants, diesel fuel, and phenol; central part of Minsk |
SV10 | Apr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries 105-kb plasmid pSVS10 | Same as SV9 |
SV11 | Tcr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries 130-kb plasmid pSVS11 | Railway bed soil contaminated with mixture of lubricants, diesel fuel, and phenol; northwestern part of Minsk |
SV12 | Apr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries 195-kb plasmid pSVS12 | Park soil with no history of known contamination; northeastern part of Minsk |
SV13 | Nalr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries 78-kb plasmid pSVS13 | Same as SV12 |
SV15 | Cmr Hgr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries 90-kb plasmid pSVS15 | Piece of rubber from storage of used car tires; central part of Minsk |
SV16 | Apr Cmr Hgr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries 123-kb plasmid pSVS16 | Sandy soil contaminated with diesel oil; building area in northeastern part of Minsk |
SV17 | Apr Tcr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries ∼200-kb plasmid pSVS17 | Sandy washout from a highway contaminated with a mixture of oil products; central part of Minsk |
SV19 | Apr Tcr Cmr Hgr Tln+ mXln+ mTol+ Ben+ C2,3O+ Sal+; carries 107-kb plasmid pSVS19 | Shoreline sediments contaminated with a mixture of oil products; Swislach River, central part of Minsk |
SV20 | Apr Hgr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries plasmid pSVS20 (>200 kb) | Same as SV19 |
SV22 | Apr Kmr Tcr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries 126-kb plasmid pSVS22 | Sandy soil contaminated with a mixture of industrial lubricants and engine oil; local landfill 10 km from the southwestern border of Minsk |
SV23 | Apr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries 130-kb plasmid pSVS23 | Same as SV22 |
SV24 | Tln+ mXln+ mTol+ Ben+ C2,3O+; carries 105-kb plasmid pSVS24 | Same as SV22 |
SV25 | Cmr Tln+ mXln+ mTol+ Ben+ C2,3O+; carries a 2-kb cryptic plasmid | Piece of road cover material; local landfill 10 km from the southwestern border of Minsk |
Apr, Tcr, Kmr, Nalr, Cmr, and Hgr, resistance to ampicillin, tetracycline, kanamycin, nalidixic acid, chloramphenicol, and HgCl2, respectively, in the concentrations given in Materials and Methods. Tln+, mXln+, mTol+, Ben+, and Sal+, ability to use toluene, m-xylene, m-toluate, benzoate, and salicylate, respectively, as sole carbon and energy sources. C2,3O+, positive for C2,3O activity.