TABLE 2.
Bacterial strains and plasmids
| Bacterial strains and plasmids | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | F−supE44 (φ80dlacZΔM15) Δ(lacZYA-argF)U169 hsdR17 recA1 endA1 gyrA96 (Nalr) thi-1 relA deoR | Gibco BRL Life Technologies |
| E. coli ED8654 | supE supF hsdR metB lacY gal trpR; host for propagation of pWW53-3001 and pWW53-3002 | P. A. Williams |
| E. coli LE392(RP1) | Plasmid of IncP1 incompatibility group; Kmr Tcr | S. Harayama |
| P. putida mt-2 | Tln+ mXln+ mTol+ Ben+ C2,3O+; host of pWW0 | P. A. Williams |
| P. putida MT53 | Tln+ mXln+ mTol+ Ben+ C2,3O+; host of pWW53 | P. A. Williams |
| P. putida AC13 | His− Rifr Smr; recipient strain for TOL plasmids | A. Boronin |
| P. putida AC34(pMG18) | Plasmid of IncP9 incompatibility group; Smr Gmr Kmr Hgr | G. Jacoby |
| Plasmids | ||
| pWW53-3001 | HindIII 17.5-kb fragment of pWW53 cloned in pKT230; contains meta pathway operon II | P. A. Williams |
| pWW53-3002 | HindIII 15.6-kb fragment of pWW53 cloned in pKT230; contains meta pathway operon I | P. A. Williams |
| pUC18 | Apr ColE1 replicon; general cloning vector | Promega Corporation |
| pUC28 | Apr ColE1 replicon; general cloning vector | Promega Corporation |
| pGEM-T Easy | Apr ColE1; linearized pGEM-5Zf(+) with single 3′ thymidine overhangs to facilitate cloning of PCR products | Promega Corporation |
| pPL392 | 16.4-kb EcoRI fragment of pWW0; contains complete meta cleavage pathway operon and xylS cloned into pBR322 | 18 |
| pCVS22 | 6.3-kb EcoRI fragment of pSVS11 cloned into pUC18; contains xylW (3′ end) and xylCMABN (5′ end) | This study |
| pCVS31 | 5.3-kb HpaI fragment of pSVS10 cloned into pUC28; contains xylC (3′ end) and xylMABN (5′ end) | This study |
| pCVS32 | 5.3-kb HpaI fragment of pSVS11 cloned into pUC28; contains xylC (3′ end) and xylMABN (5′ end) | This study |
| pCVS42 | 5.5-kb HpaI fragment of pSVS10 cloned into pUC28; contains xylS (5′ end), xylR, interoperonic area, xylU, and xylW (5′ end) | This study |
a
Rifr, Smr, and Gmr, resistance to rifampin, streptomycin, and gentamicin, respectively. His−, histidine negative. For other abbreviations, see Table 1, footnote a.