A phylogenetic tree of Argonaute (AGO) proteins in green plants. The tree was constructed using dataset I implemented in RAxML. Dataset I includes 187 AGO proteins from 3 chlorophytes, green; 13 charophytes, blue; 5 bryophytes, orange; 1 lycophyte, purple, 2 ferns, light blue; 5 gymnosperms, red; 4 angiosperms, black. The sequences from chlorophytes (Chlamydomonas reinhardtii, Volvox carteri and Chromochloris zofingiensis) are clustered and named Chlorophytic AGO. The AGO1/5/10, AGO2/3/7, AGO4/6/8/9 clades were named after the 10 Arabidopsis AGOs, and the AGO-like clade was named according to You et al. (2017). AGOs from charophytes, bryophytes, lycophytes, ferns, gymnosperms and angiosperms are present in all clades except for the AGO-like clade. The inner circle (black bars) is identified AGO subclade. The numbers next to the nodes are bootstrap support values (from 0 to 100).