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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Plant J. 2021 Dec 14;109(5):1086–1097. doi: 10.1111/tpj.15615

Figure 3.

Figure 3.

Phylogenetic relationship and multiple sequence alignment of the DEDD/H motif in the AGO4 clade. To aid presentation, some representative species from each order were included. The topology shows that AGO4 in eudicots can be clearly classified into three sub-groups: Eudicots-Group I; Eudicots-Group II; and Eudicots-Group III. Residues in the catalytic tetrad DEDD/H (Asp-Glu-Asp-Asp/His) motif are indicated by black rectangles, and AGO-II members that do not possess a canonical ’DEDH/D’ motif are indicated with the red rectangle. The numbers at the top of the sequence alignment are the positions of amino acids. The numbers next to the nodes are bootstrap support values (from 0 to 100). AGO, Argonaute.