Phylogenetic relationship within the AGO10 clade. To aid presentation, only selected species were included to represent each order. The topology shows that AGO10 in eudicots can be clearly classified into two clades, AGO10a and AGO10b. Each of them includes all major lineages in angiosperms: basal angiosperms, purple; monocots, blue; magnoliids, red; eudicots, green. Most commercial crops contain members of both clades, such as Glycine max, Vitis vinifera and Gossypium raimondii, etc. However, no Arabidopsis AGO10 is found in AGO10b and no Oryza AGO10 is in AGO10a. The numbers next to the nodes are bootstrap support values (from 0 to 100). AGO, Argonaute.