FIG. 7.

Modest concentration of GSK3 inhibitor plus p38 inhibitor promotes the expansion of human UCB HSCs. MNCs were collected from 2 U of UCB separately and were cultured in stem cell medium with 0.5 μM SB, 0.5 μM CHIR, or a combination of both for 12 days with medium change every 3 days. (A, B) The percentages and absolute numbers of CD34⁺ and CD34⁺CD38⁻ HSCs were determined by FACS. (C) The LT-HRC of the cultured cells was analyzed by CHR assay. UCB, umbilical cord blood. Color images are available online.