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. Author manuscript; available in PMC: 2022 Jun 20.
Published in final edited form as: Clin Exp Allergy. 2020 Nov 25;51(2):318–328. doi: 10.1111/cea.13776

TABLE 2.

Association of DNA methylation (DNAm) change with asthma acquisition from pre- to post-adolescence at 13 cytosine-phosphate-guanine (CpG) sites that are sex-specific. Significant interactions between DNAm and sex on asthma acquisition from 10 to 18 y identified in the Isle of Wight birth cohort (IOWBC)a were further tested in the Avon Longitudinal Study of Parents and Children 11(ALSPAC) cohortb. Males are in the reference group

CpG site Est.c IOWBC Gene CpG islands Gene location Chr.e ALSPAC cohort
Int.c 95% CIc P dRaw P dFDR Est.c Int.c P d Raw
cg03269757 −1.34 4.38 1.93, 6.83 1.88 × 10−4 .02 ATL2 N_Shore Body 2 0.03 0.59 .19
cg11295724 −0.33 7.82 4.12, 11.52 5.35 × 10−6 .003 SIRPD – TSS200 20 0.06 0.76 .22
cg12587133 0.34 4.85 2.37, 7.32 1.03 × 10−5 .003 AGA – 4 0.01 0.24 .5
cg15154628 −0.67 3.23 1.14, 5.31 1.15 × 10−3 .05 CCDC146 – Body 7 0.24 0.07 .89
cg16301989 −0.48 4.42 1.89, 6.93 1.86 × 10−4 .02 FUNDC2P2f – 2 0.05 0.47 .26
cg18278943 0.70 4.55 1.87, 7.24 4.36 × 10−4 .03 SLMAP N_Shelf 3 −0.25 0.82 .18
cg20885063 −0.52 4.45 1.86, 7.05 3.78 × 10−4 .03 ATPAF2 N_Shelf Body 17 0.007 0.4 .36
cg20891917 −0.43 5.5 2.03, 8.96 9.86 × 10 −4 .04s IFRD1 – 5′UTR 7 −0.13 1.26 .01
cg21163444 −2.22 11.13 4.31, 17.95 8.53 × 10−4 .04 ZNF385A S_Shore Body 12 0.27 0.14 .86
cg22484084 0.29 7.18 2.69, 11.67 1.0 × 10−3 .04 PTPRV – TSS1500 1 0.13 0.84 .39
cg06492287 −1.91 10.49 4.73, 16.25 7.78 × 10−5 .01 SNTG2 – Body 2 0.002 −0.04 .96
cg11770323 −0.7 9.18 4.11, 14.25 7.92 × 10−5 .01 NDFIP2 – Body 13 −0.75 −0.55 .29
cg11814087 0.58 6.14 2.29, 10.01 8.85 × 10−4 .04 ZFR – Body 5 0.11 −0.68 .05
a

For the analyses in IoW, logistic regression models were adjusted for birth weight, sex, maternal and paternal disease status of asthma, socio-economic status (SES), change of atopic status, BMI, height from 10 to 18 y.

b

Analyses of ALSPAC used similar covariates: maternal disease status of asthma, sex, birth weight, SES, atopy status at 7 y, and changes of height, and BMI from 7 to 15/17 y.

c

Est., Estimated main effect; Int., interaction coefficient of the CpGs with the sex of a child. Interaction effects consistent between the two cohorts are with bold fonts; CI, confidence interval.

d

PRaw, raw P-value; PFDR, adjusted P-value for multiple testing by controlling a false discovery rate (FDR) of .05. All the P-values are for interaction effects.

e

Chr., Chromosome.

f

Genes closest to the CpG site annotated using the UCSC genome browser.