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. 2022 Apr 7;13(4):9302–9312. doi: 10.1080/21655979.2022.2051854

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — HCP5 regulates HK2 mRNA stability via m⁶A. A, B. qRT-PCR and western blot analyzing the indicated RNA and protein levels in ESCC cells with HCP5 knockdown. C. Luciferase reporter assay testing the effect of HCP5 knockdown on HK2 promoter. D. qRT-PCR testing the half-life of HK2 mRNA after HCP5 knockdown. E. qRT-PCR testing HK2 mRNA level in HCP5-silenced ESCC cells overexpressed METTL3. F. RNA pull-down assay testing the binding of HCP5 to METTL3 and YTHDF1. G, H. RIP assay testing the binding of YTHDF1 to HCP5 and HK2 mRNA. I. MeRIP assay testing the m⁶A level on HK2 mRNA. *P < 0.05.