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. 2022 Mar 29;13(3):6243–6256. doi: 10.1080/21655979.2021.2003929

Figure 3.

Figure 3.

Knockout miR-485-5p expedites TAM M2 polarization, and CRC EMT and glycolysis a. RT-qPCR to detect miR-485-5p in CRC and adjacent normal tissues; b. RT-qPCR to detect miR-485-5p in CRC cell line SW480 and human normal colonic epithelial cell line NCM460; c. RT-qPCR to detect miR-485-5p in SW480 cells transfected with miR-485-5p inhibitor; d. Colony formation detection of the impact of miR-485-5p suppression on SW480 cell proliferation; e. Flow cytometry to detect the impact of miR-485-5p suppression on apoptosis of SW480 cells; f. Flow cytometry to detect the impact of macrophages cultured with SW480 cells with silenced miR-485-5p and CM on the proportion of CD206+ and CD163+ cells; g. RT-qPCR to detect the impact of macrophages cultured with SW480 cells with silenced miR-485-5p and CM on M1 macrophage markers TNF-α and IL-6 and M2 macrophage markers IL-10 and MRC1; h. The impacts of suppressive miR-485-5p on glucose consumption, lactic acid and pyruvate production of SW480 cells; I. Western blot to detect the impacts of miR-485-5p suppression on E-cadherin, N-cadherin and Snail in SW480 cells. Expression of the data was as mean ± SD (n = 3); *P < 0.05.