Figure 2.

Effect of Livogrit on development of NASH on monolayer culture of rat primary hepatocyte. (a) Cytosafety analysis (72 h) post Livogrit (0–100 µg/mL) and Pioglitazone (10 µM) treatment, as determined by Alamar blue assay (Ex 560/Em 590 nm). (b) Intracellular lipid accumulation (72 h) post incubation in MCDM with treatment of Livogrit (0, 0.3, 3, 30 µg/mL) and Pioglitazone (10 µM), as determined by Nile red stain (Ex 530/Em 600 nm) fluorescence measurement. (c) Fluorescent microscopy images of Hoechst 33342 (DAPI) and LipidSpot 610 (Cy5) stained rat primary hepatocytes post (72 h) incubation in MCDM with treatment of Livogrit (0, 30 µg/mL) and Pioglitazone (10 µM). Scale bar = 20 µm.