Skip to main content
. 2022 May 3;13(4):9135–9147. doi: 10.1080/21655979.2021.2003942

Figure 6.

Figure 6.

Repression of OS via elevated miR-22-3p is reversed via simultaneous overexpression of TCF7L2A. RT-qPCR and Western blot were applied to detect miR-22-3p and TCF7L2 in MG63 and HOS cells after transfection with miR-22-3p-mimic and pcDNA 3.1-TCF7L2; B. CCK-8 to detect MG63 and HOS cell proliferation after transfection with miR-22-3p-mimic and pcDNA 3.1-TCF7L2; C. Flow cytometry to detect the apoptosis rate of MG63 and HOS cells transfected with miR-22-3p-mimic and pcDNA 3.1-TCF7L2; D. Cell scratch for detection of MG63 and HOS cell migration after transfection with miR-22-3p-mimic and pcDNA 3.1-TCF7L2; E. Transwell for detection of MG63 and HOS cell invasion after transfection of miR-22-3p-mimic and pcDNA 3.1-TCF7L2; F. Western blot for detection of Wnt and β-catenin expression in MG63 and HOS cells transfected with miR-22-3p-mimic and pcDNA 3.1-TCF7L2. The values were shown as mean ± SD (n = 3). The significance of each group was calculated using one-way ANOVA, and the variance correction via Tukey’s test. Vs. the miR-22-3p-mimic+pcDNA 3.1 group, * P < 0.05.