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Arctiin elevated bone formation in MC3T3-E1 cells. (a) Cell viability was determined with a CCK-8 assay after the transfected or non-transfected MC3T3-E1 cells (2 × 103 cells/well) treatment with various concentrations of Arctiin (2.5, 5, 10 and 20 μM) for 48 h. (b) RT-qPCR and (c) western blot were employed to examine the expression of osteogenesis markers including RUNX2, COL1A1, OCN and OPN. **P < 0.01, ***P < 0.001.
