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Ccnd1 downregulation attenuated the impacts of Arctiin on bone formation in MC3T3-E1 cells. (a) The expression of Ccnd1 was determined with western blotting after transfection with shRNA targeting Ccnd1. (b) The mRNA expression of RUNX2, COL1A1, OCN and OPN was detected using RT-qPCR. (c-d) Western blot analysis was adopted for the assessment of RUNX2, COL1A1, OCN and OPN proteins expression. *P < 0.05, **P < 0.01, ***P < 0.001.
