Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 16;13(3):7904–7918. doi: 10.1080/21655979.2022.2052673

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — SK induces apoptosis and autophagy in HCC cells. a. Apoptosis was detected by annexin V/PI double staining. The results are expressed as a percentage of the control, which is set to 100%. b. The LC3 puncta were examined using confocal microscopy and were quantified. LC3 is shown in green. DAPI, shown in blue, stained the nuclei. Confocal microscope was taken at ×20. c. After being treated with different concentrations of SK for 48 hours, apoptosis (RAPA, caspase3, caspase9, Bax, Bcl2), autophagy (p-ULK, ULK, Bclin1, p62, LC3) and pathway (p-PI3K, PI3K, p-Akt, Akt, p-mTOR and mTOR related proteins expression was analyzed by Western blot. The data are expressed as the mean ± standard deviation of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.