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. 2022 Mar 16;13(3):7904–7918. doi: 10.1080/21655979.2022.2052673

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The Anticancer effects of Knockdown of PYCR1 in HCC cells. a. Relative levels of PYCR1 expression in LO2, SMMC-7721, Huh-7, Hep-3B, SNU-449, BEL-7404, and HepG2 were examined via RT-qPCR and Western blot. b. HCC cells were transfected with siRNA and the expression level of PYCR1. c. The CCK8 assay was employed to detect the viability of HCC cells. d. Colony formation assay was performed in SNU-449and Hep-3B cells treated with SK. e. Migration and invasion of HCC cells treated with SK for 24 hours by Transwell assay. f. The wound-healing assay obtained images at 0 and 24 hours after scratch. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.