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Downregulation of PYCR1 induces apoptosis and autophagy in HCC cells. a. SNU-449and Hep-3B cells expressing negative control or siPYCR1. LC3 signal was detected under a confocal microscope. The LC3 signal quantity of each cell was quantified. Scale bar: 100 μm. b. Apoptosis was detected by flow cytometry. c. Western blot analysis was used to determine the protein levels in both SNU-449and Hep-3B cells. * P < 0.05, ** P < 0.01, *** P < 0.001 vs the control(n = 3).
