Figure 6.

SK induced apoptosis and autophagy of HCC cells by downregulated PYCR1 via inhibited PI3K/Akt/mTOR pathway. a. Immunofluorescence assay (× 20) detected LC3 puncta of SNU-449and Hep-3B cells treated with SK and siPYCR1transfection for 24 h. b. The apoptosis of SNU-449and Hep-3B cells was detected by flow cytometry. c. The expression of pathway protein was detected by Western blotting, with β- Actin, was used as a loading control. Data are presented as the mean ± SD of three independent experiments (n = 3, * P < 0.05 for SK vs CON or NC, #P < 0.05, SK +siPYCR1 vs SK).