Figure 6.

CRISPR/dCas9-based engineering of the epigenome. The techniques are based on using inactive-Cas (dCas) to allocate the desired protein to the target sequence. For DNA methylation, cytosine methylation could be used by linking dCas with DNMT3A or MQ1 (a), while demethylation of cytosine could be edited by linking Tet1 with dCas (b). Chromatin modifiers could be edited either by acetylation/deacetylation using HDAC/HAT or methylation/demethylation using HMT/HDM with dCas9.