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. 2021 Jul 29;17(3-4):56–71. doi: 10.1080/15476278.2021.1954769

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Figure 2. — Dynamic co/monoculture versus static co/monoculture effects on metabolic activity and ALP activity of hASCs. hASCs were pre-treated for 30 min with 1,25-(OH)₂VitD₃ and cocultured with HUVECs in a modified perfusion bioreactor (dynamic culture) and flask bioreactor (static culture) for 14 days. (a) Static coculture, dynamic coculture, static monoculture and dynamic monoculture increased proliferation by 2.79-fold, 2.91-fold, 2.98-fold, and 3.11-fold, respectively. (b) Dynamic coculture increased ALP activity by 7.1-fold compared to static coculture and dynamic monoculture increased ALP activity by 24.1-fold compared to static monoculture. Values are mean ± SEM (n = 3). ALP, alkaline phosphatase; hASCs, human adipose stem cells; 1,25-(OH)₂VitD₃, 1,25-dihydroxyvitamin D₃. HUVEC, human umbilical vein endothelial cells. *Significantly different from static culture, p < .05.