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. 2021 Jul 29;17(3-4):56–71. doi: 10.1080/15476278.2021.1954769

Figure 5.

Figure 5.

Dynamic co/monoculture versus static co/monoculture effects on osteogenic gene expression of hASCs and angiogenic gene expression of the accumulation of cells on PLGA/β-TCP/PCL scaffold. Pre-treated hASCs with 1,25-(OH)2VitD3 cocultured with HUVECs in static and dynamic culture for 14 days. Dynamic coculture increased (a) Ki-67 expression by 6.8-fold; decreased (b) RUNX2 by 0.55-fold, (c) OPN by 0.71-fold, (d) SPARC by 0.33-fold, and (e) OCN by 0.71-fold; increased (f) CYP27B1 by 5-fold (g) VEGF165 by 6-fold, (h) VEGF189 by 2-fold, and (i) Endothelin-1 by 4-fold compared to dynamic monoculture. Values are mean ± SEM (n = 3). PLGA, poly(lactic-co-glycolic acid); β-TCP, β-Tricalcium phosphate; PCL, Polycaprolactone; Ki-67, proliferation marker; RUNX2, Runt-related transcription factor 2; OPN, osteopontin; SPARC, osteonectin; OCN, osteocalcin; CYP27B1, cytochrome P450 family 27 subfamily B member 1; VEGF, vascular endothelial growth factor. *Significantly different from dynamic monoculture, p < .05.