TABLE 3.
Digestion of purified cellulose by rumen contents in media without additions or with antibiotics, cycloheximide, or both after an initial 0- or 18-h fermentation and autoclaving
| Initial incubation (h)a | Amt of cellulose digested in initial incubation (mg) | Amt of cellulose digested (mg) in second fermentation (72 h)b
|
|||
|---|---|---|---|---|---|
| Control | Antibiotics | Cycloheximide | Antibiotics + cycloheximide | ||
| 0 | 0 | 112.6 ± 4.2 (59.1)c | 95.4 ± 16.0 (50.7) | ||
| 18 | 39.1 ± 10.8 (20.0) | 73.8 ± 10.0A (59.2) | 21.4 ± 4.4B (31.4) | 68.8 ± 8.1A (56.6) | 0 |
a
All tubes were autoclaved for 20 min at 121°C after the initial incubation time and then inoculated again with rumen contents for the second fermentation.
b
Means in the same row without a common letter differ (P < 0.01).
c
Total percent of cellulose digested in all incubations, single or combined where applicable.