TABLE 6.

Water solubility of the inhibitory activity in LRF^a

Addition to antibiotic-containing medium	Cellulose digestion (%) after incubation periodbc
	Trial 1		Trial 2 (7 days)
	5 days	14 days
None	31.2 ± 2.5A	37.8 ± 2.9B	41.0 ± 5.3B
LRFd	11.4 ± 1.3BC	3.2 ± 2.1DE	NDe
Water insoluble	43.8 ± 4.3E	45.2 ± 2.5B	58.6 ± 3.6E
Water soluble	2.6 ± 1.7F	3.0 ± 1.9E	13.7 ± 1.6F

^aEach value is based on six fermentation tubes (three replicates in duplicate). 

^bMeans in the same row without a common letter differ at P < 0.01 for C and D and means in the same column without a common letter differ at P < 0.05 for A, B, E, and F. 

^cMean ± standard error. 

^dLRF added at a level equivalent to 100% rumen fluid in the medium. 

^eND, not determined.