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. 2021 Jul 27;29(6):734–749. doi: 10.1038/s41417-021-00352-2

Fig. 2. EVs derived from NK-92 cells are taken up by recipient cells via macropinocytosis.

Fig. 2

A K562 cells were incubated with PBS or 5 μg of PKH67-labeled NK-EVs for 3, 6, or 24 h at 37 °C. The percentage of PKH67+ K562 cells was determined by flow cytometry. The graphs represent the means + SEM of the percentage of PKH67+ K562 cells. Numbers indicate the percentage of PKH67+ cells. B K562 cells were incubated with PBS or 5 μg of PKH67-labeled NK-EVs for 24 h at 37 °C. Nuclei were stained with DAPI (scale bars, 20 μm). C K562 cells were incubated with PBS or 5 μg of PKH67-labeled NK-EVs for 3 or 6 h at 37 or 4 °C. The percentage of PKH67+ K562 cells were analyzed by flow cytometry. Viablility of cells was approximately 95% after incubation for 3 or 6 h at 4 °C. The graphs show the means + SEM of the percentage of PKH67+ K562 cells. D K562 cells were preincubated with DMSO or 75 μM EIPA for 30 min. Cells were then incubated with PBS or 5 μg of PKH67-labeled NK-EVs for 3 or 6 h. The percentage of PKH67+ K562 cells were analyzed by flow cytometry. The graphs represent the means + SEM of the percentage of PKH67+ K562 cells. Cell cultures were performed in triplicate. All data shown are representative of three independent experiments. ****P < 0.0001.