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. 2022 Jan 28;18(5):1834–1850. doi: 10.1007/s12015-022-10335-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The hUC-MSC-sec promoted primordial follicle activation in vitro. A. After 12 days of in vitro culture, the histological analysis of hematoxylin and eosin staining showed more activated follicles in hUC-MSC-sec-treated ovaries than controls. The arrowheads indicate primordial follicles, and the arrows indicate activated follicles. B. Quantification of ovarian follicles showed no obvious difference in the total number of follicles between hUC-MSC-sec-treated ovaries (5510 ± 832.2) and controls (5723 ± 825.3). C. Ovarian follicle counts revealed a significantly increased proportion of activated follicles in hUC-MSC-sec-treated ovaries (52.4 ± 5.3%) compared to controls (25.5 ± 2.3%). Data are shown as the mean ± SD, n = 6. ***P < 0.001. Scale bars, 100 μm