Fig. 2.

The hUC-MSC-sec activated the PI3K-AKT signaling pathway. A. There was a time-dependent increase in p-AKT level in hUC-MSC-sec-treated ovaries after 1, 2, and 4 days of in vitro culture compared to controls. β-Actin was used as the internal control. B. The localization of FOXO3a (red fluorescence) in oocyte cytoplasm (DDX4, green fluorescence) was increased in MSC-sec-treated ovaries after 4 days of culture compared to controls. Nuclei were counterstained with Hoechst 33342 (blue fluorescence). The arrowheads indicate nuclear localization of FOXO3a, and the arrows indicate the cytoplasmic localization of FOXO3a. C. The proportion of cytoplasmic localization of FOXO3a (CL-FOXO3a) was significantly increased in MSC-sec-treated ovaries (43.0 ± 4.7%) compared to controls (31.8 ± 4.6%). Data are shown as the mean ± SD, n = 6. ***P < 0.001. Scale bars, 100 μm