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. 2022 Jan 28;18(5):1834–1850. doi: 10.1007/s12015-022-10335-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — HGF promotes the activation of primordial follicles via KITL. A. After 12 days of in vitro culture, the histological staining showed more activated follicles in HGF-treated ovaries than controls. The number of activated follicles was decreased in the HGF plus KITL^ab group compared to the HGF group. The arrowheads indicate primordial follicles, and the arrows indicate activated follicles. B. Quantification of ovarian follicles showed no obvious differences in the total follicle count among the control group (7588 ± 1408), the HGF-treated group (7533 ± 945), and the HGF plus KITL^ab-treated group (6472 ± 866.4). C. Quantification of ovarian follicles showed a significantly increased proportion of activated follicles in HGF-treated ovaries (61.6 ± 5.0%) compared to controls (46.0 ± 4.5%) and a significantly decreased proportion in HGF plus KITL^ab-treated ovaries (48.1 ± 6.2%) compared to HGF-treated ovaries. Data are shown as the mean ± SD, n = 6. **P < 0.01, and ***P < 0.001. Scale bars, 50 μm